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Nevertheless, it is clear that the PDE11A variants demonstrate differential tissue expression. In humans, PDE11A1 mRNA is most prominent in skeletal muscle and prostate (Fawcett et al., 2000; Yuasa et al., 2001a). PDE11A3 mRNA is found specifically in testis and PDE11A4 mRNA is highly expressed in prostate (Yuasa et al., 2000). PDE11A protein localization studies have been somewhat contradictory in their findings, probably because of differences in the specificity of the antibodies used. PDE11A1 protein was originally detected in prostate and skeletal muscle (Fawcett et al., 2000), although a later study did not detect PDE11A1 protein in any tissues (Loughney et al., 2005). In fact, only PDE11A4 protein has been verified and is found in prostate, pituitary, heart, and liver. Another study suggested that PDE11A is widely expressed, and immunohistochemistry using an antibody reported to recognize all PDE11A variants localized it to the epithelial, endothelial, and smooth muscle cells of many tissues, but at highest levels in the prostate, testis, kidney, adrenal gland, colon, and skin (D'Andrea et al., 2005). However, a separate study did not find any PDE11 protein expression in human testis (Loughney et al., 2005). As with many PDEs, it is still not clear if the same tissue, cellular, and subcellular localization is found among species. Future work will hopefully rectify the discrepancies regarding the tissue expression of PDE11A. branded cialis 20mg using viagra when you don't need it Dr. John R. Brinkley initiated a boom in male impotence cures in the US in the 1920s and 1930s. His radio programs recommended expensive goat gland implants and "mercurochrome" injections as the path to restored male virility, including operations by surgeon Serge Voronoff. After the Kansas State Medical Board revoked his medical license and the Federal Radio Commission refused to renew his radio license (both in 1930), Brinkley moved his operations just over the Texas border to Mexico where he opened a medical clinic and broadcast advertisements into the US from a border blaster radio station. Erections depend on healthy blood flow to the penis. During an erection, vessels in the penis become engorged with blood to produce an erection. Many diseases that affect the blood vessels can interfere with blood flow to the penis. Atherosclerosis (hardening of the arteries) and high blood pressure (hypertension) can cause abnormal blood flow to the penis and can affect a man's ability to have an erection. canadian brand name viagra us generic viagra bodybuilding pills Failure to achieve an erection less than 20% of the time is not unusual and treatment is rarely needed. 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